Formulation of Steinernema yirgalemense in gel for long-term storage at room temperature

Abstract The use of entomopathogenic nematodes (EPNs) offers the potential reducing chemical insecticides in agricultural industry. Hydrogels for long-term storage EPNs may have to improve ability EPNs. In this study, infective juveniles (IJ) a South African isolate Steinernema yirgalemense were formulated different gels, which included laboratory grade and consumer agar, as well STOCKOSORB® 660, compared it with IJ water. Their survival pathogenicity investigated at 25 °C. last instar larvae Tenebrio molitor used test IJs 6 weeks postformulation. stored 1% 1.5% agar formulations found provide best 77% 82% after formulation. Results indicate be comparable can cheaper alternative formulation S. . When comparing 660 formulations, 80% was recorded 1 g 50 ml water, 50% surviving 100 gel more virulent than agar.